LCSM – Lin’s Cupric Sulfate Medium

LCSM is used for the detection and quantitative determination of wild yeast populations in brewing culture yeast.  It is designed to encourage the growth of non-Saccharomyces yeast.  An inoculum of approximately 1 million culture yeast cells needs to be plated on LCSM.  While the growth of culture yeast will be suppressed by cupric sulfate, wild yeast will develop as large, distinct colonies.  A few Saccharomyces, like S. cerevisiae var. diastaticus, may be more resistant to cupric sulfate and show a certain level of growth.

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